S. elongatus PCC 7942 Carbon Metabolism Proteomics (MC-DP2)

The purpose of this experiment was to examine the redox proteome of S. elongatus PCC 7942 CscB/SPS under different light conditions (light and dark) and culture conditions (dense and dilute optical density). Samples were processed using a resin-assisted capture (RAC) workflow with TMT labeling to enrich and quantify protein cysteines which were differentially oxidized under four conditions. The datasets were generated by a Q Exactive Plus Orbitrap Mass Spectrometer coupled with a Waters nanoAcquity UPLC, then searched by MSGF+ for downstream redox PTM analysis.. DOE Contract: AC05-76RL01830. Subjects: 59 BASIC BIOLOGICAL SCIENCES; Predictive Phenomics, S. elongatus PCC 7942, Proteomics, Tandem Mass Tag 16-Plex, global analysis, post translational modifications, central carbon metabolism, circadian rhythm